PCR試劑 腸道病毒/帕氏病毒/腺病毒核酸檢測試劑盒

廣州健侖生物科技有限公司

準(zhǔn)備使用lyo master混合物（每個(gè)8孔條）檢測腸道病毒，帕氏病毒和腺病毒包括內(nèi)部對照。
Ready to use lyo master mix (8-well strips each) for detection of enterovirus, parechovirus and adenovirus including internal control.
Principle
Multiplex real-time PCR for detection of pathogen genes by TaqMan® technology
Targets
Lyo mastermix:
enterovirus
human parechovirus
human adenovirus
internal control
Specimen
This test is for use with extracted nucleic acid from CSF, blood (with heparin), throat swabs, sputum and stools of human origin
Storage
Liquid components: -20°C
Lyophilised components: 2-8°C
Sealing foils: room temperature
Shelf life
12 months from manufacture

PCR試劑 腸道病毒/帕氏病毒/腺病毒核酸檢測試劑盒

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

  （1）高壓熱修復(fù) 在沸水中加入EDTA（pH8.0）或0.01M枸櫞酸鈉緩沖溶液（pH6.0）。蓋上不銹鋼高壓鍋的蓋子，但不進(jìn)行鎖定。將玻片置于金屬染色架上，緩慢加壓，使玻片在緩沖液中浸泡5分鐘，然后將蓋子鎖定，小閥門將會升起來。10分鐘后，去除熱源，置入涼水中，當(dāng)小閥門沉下去后打開蓋子。本方法適用于較難檢測或核抗原的抗原修復(fù)。
   （2）煮沸熱修復(fù) 電爐或者水浴鍋加熱0.01M枸櫞酸鈉緩沖溶液（pH6.0）至95℃左右，放入組織芯片加熱10~15分鐘。
   （3）微波熱修復(fù) 在微波爐里加熱0.01M枸櫞酸鈉緩沖溶液（pH6.0）至沸騰后將組織芯片放入，斷電，間隔5~10分鐘，反復(fù)1-2次。適用的抗原有：AR，Bax，Bcl-2，C-fos，X-jun，C-kit，C-myc，E-cadherin，Chromogranin A，Cyclin，ER，Heat shock protein，HPV，Ki-67，MDMZ，p53，p34，p16，p15，P-glycoprotein，PKC，PR，PCNA，ras，Rb，TopoismeraseⅡ等。是以高溫，高壓對常規(guī)固定的石蠟切片進(jìn)行抗原修復(fù)或復(fù)原的一種非蛋白酶消化以提高抗原抗體陽性檢測的一種方法和技術(shù)手段。甲醛和蛋白水解交聯(lián)過程中氨基酸側(cè)鏈上的某些基團(tuán)(抗原決定簇)如咪唑、吲哚等基團(tuán)受到影響，通過120℃高溫或強(qiáng)堿處理后，可使交聯(lián)打開。
   2）酶消化方法 常用0.1%胰蛋白酶和0.4%胃蛋白酶液。胰蛋白酶使用前預(yù)熱至37℃，切片也預(yù)熱至37℃，消化時(shí)間約為5~30分鐘；胃蛋白酶消化37℃時(shí)間為30分鐘。適用于被固定遮避的抗原，其中有：Collagen，Complement，Cytokeratin，C-erB-2，GFAP，LCA，LN等。

(1) High-pressure heat repair Add EDTA (pH8.0) or 0.01M sodium citrate buffer solution (pH6.0) to boiling water. Cover the stainless steel autoclave lid without locking. Place the slide in a metal-stained rack, apply pressure slowly and allow the slide to soak for 5 minutes in the buffer, then lock the lid and the small valve will lift. After 10 minutes, remove the heat and place in cold water and open the lid when the small valve is lowered. The method is more difficult to detect antigen or antigens antigen.
   (2) boil hot repair electric furnace or water bath heating 0.01M sodium citrate buffer solution (pH6.0) to about 95 ℃, into the tissue chip heating 10 to 15 minutes.
   (3) Microwave Heat Repair In the microwave oven heating 0.01M sodium citrate buffer solution (pH6.0) to the chip after boiling into the tissue, power, interval of 5 to 10 minutes, repeated 1-2 times. Antigens such as AR, Bax, Bcl-2, C-fos, X-jun, C-kit, C-myc, E-cadherin, Chromogranin A, Cyclin, ER, Heat shock protein, MDMZ, p53, p34, p16, p15, P-glycoprotein, PKC, PR, PCNA, ras, Rb and Topoismerase II. Is a high temperature, high pressure on the routine fixed paraffin sections antigen retrieval or recovery of a non-protease digestion to improve antigen-antibody positive detection of a method and technical means. Formaldehyde and proteolytic crosslinking some of the amino acid side chains (epitopes) such as imidazole, indole and other groups are affected, through 120 ℃ high temperature or alkali treatment, you can make the cross-link open.
   2) enzyme digestion method commonly used 0.1% trypsin and 0.4% pepsin solution. Trypsin was preheated to 37 ° C before use and the pellet was also preheated to 37 ° C with a digestion time of about 5 to 30 minutes and pepsin digestion at 37 ° C for 30 minutes. It is suitable for antigens that are fixedly sheltered and include: Collagen, Complement, Cytokeratin, C-erB-2, GFAP, LCA, LN and others.